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DNA Lab Equipment Fund
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A project fund forCitizen Mycology

$1,872 given of $4,552 goal

DNA Lab Equipment Fund

Started by: Amanda Schabdach

8 shares

  • Website
    Post Campaign to a Blog/Website

This campaign exists to fund all aspects of the DNA prep work for interesting specimens we collect in the Nantahala Rainforest. This includes all equipment, tools, and chemical reagents necessary to extract and purify the DNA from fungal samples. Purchase any item from our wish list to donate to our citizen mycology laboratory directly! Our greatest hope is this campaign will give us molecular biology capabilities. This project is being run by a fully qualified female citizen scientist with a Masters in Biochemistry alongside her partner, a field mycologist and macroscopic identification wizard.

Wishlist

Set of 4 Pipettors

For moving tiny amounts of liquids with accuracy and precision. The 4 that we need are included in the full price.

$1,079.00

completed

Pipettor Stand

Stand to hold multiple pipettors, so that the tips remain free from contamination

$35.00

1 left

PCR Tubes

Tubes that go into the thermal cycler, in which the DNA polymerase reaction is preformed

$159.00

1 left

Thermal Cycler

Machine that allows the PCR reaction to run, which makes thousands of copies of ("amplifies") the DNA

$620.00

1 left

Gel Electrophoresis Platform

Allows us to check that the reactions worked before we send the samples away for sequencing

$100.00

completed

High-Performance UV Transilluminator

For illuminating the results of the gel

$699.00

1 left

Plastic tube racks

Racks to hold many plastic tubes

$12.00

2 left

Case of Nitrile Gloves

Gloves for sterile lab work

$250.00

1 left

Rotating Plate Shaker

Keeps the liquid moving so that the mixture is uniform, allows any reactions to proceed overnight if necessary

$195.00

1 left

Heat Block

Used as an analog method of heating tubes or holding them at temperature

$120.00

1 left

Ice Bucket

If many wells are necessary, the ice bucket will help to slow the reaction so all wells may proceed with uniformity

$30.00

1 left

Thermometer

To measure temperature of the heating block

$22.00

completed

Plastic Pestles

Pack of 100 plastic pestles for grinding

$72.00

1 left

Sodium hydroxide

Ingredient in master mix and buffers

$19.00

completed

Potassium Chloride

Buffer component

$21.00

completed

Tris buffer

Concentrated buffer base

$27.00

1 left

Bovine serum albumin, lyophilized powder, pro

For stabilizing the DNA in solution

$58.00

1 left

Lab Grade Glycerin

$32.00

1 left

Agarose

The material mixed with water is used to construct a gel (agarose gel)

$38.00

4 left

SYBR™ Safe DNA Gel Stain

For staining DNA in the gel

$116.00

completed

Ultra high purity water

$65.00

1 left

TAQ Polymerase

For the polymerase chain reaction in the thermo cycler (1,000 reactions)

$393.00

completed

dNTP Mix

Blank DNA, singular nucleotides combined in a mix

$152.00

1 left

General Contribution

Help with your contribution!

$10.00

9 left

11 Supporters

  • First Last (Not First? Sign Out)

  • Martha Penn2 weeks ago Love you Mandie - from GMom!
  • Mary Schabdach2 weeks ago
  • Jayden grahlman3 weeks ago Blessings of abundance my friends!!
  • Vince3 weeks ago
  • Ryan Paul Gates3 weeks ago
  • Anonymous3 weeks ago Yo, like, umm....I love you!
  • Farrah Ashlee Hibler3 weeks ago How exciting! Good luck!
  • Rebekka3 weeks ago
  • Carol Stodola3 weeks ago
  • Anonymous3 weeks ago
  • Show 1 more supporter

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